superior lab & processing


You only have one chance to save your baby's newborn stem cells.

Superior Lab Processing

Our lab utilizes a manual processing method known as the modified Rubinstein method. Typically, it yields more and healthier stem cells. Manual processing lets the cryobiologist customize the stem cell isolation phase based on the precise volume collected in the hospital. This results in more stem cells preserved and less damage to them when compared to most other methods. 

It has also been shown to yield less red blood cell contamination, less toxicity, and a significant decrease in complications when the sample is used. AlphaCord provides the most innovative processing methods, helping to guarantee the safety and viability of your newborn’s stem cells.



Many believe manual processing is superior to automated processing. It can yield higher stem cell recovery rates and reduced red blood cell contamination. With manual processing, the volume of cord blood collected is calculated and matched to the appropriate amount of reagents needed to preserve the maximum number of stem cells. Our lab has been able to successfully process and store as little as 10mL's and as much as 200mL's. Since every sample size is unique and varying in size, it is important to have the best processing method in place for each variation of volume that is received.


Automated processing requires a minimum sample size of 30-40 mls and a maximum of 150 mls. If more or less is received at the lab, the automated system is not designed to accurately account for these volume variations. Every sample size is unique. Manual processing is best suited for processing variations in volumes.


 Cord Blood

After the birth of your child, an express medical courier will be dispatched to your hospital to pick up your kit. Upon arrival at our lab, a specially trained cryobiologist will test and process your newborn's cord blood and tissues using our advanced processing methods. The birth mother's blood is thoroughly tested to ensure there are no infectious diseases present at the time of collection that could have been transferred to the samples.

The mononuclear cells found in the cord blood are separated. This is done by adding hetastarch to the sample during the sedimentation phase. Next, more unwanted blood components are removed during centrifuging. Afterward the volume of the sample will be reduced to about one quarter of what was originally collected or roughly 25 ml. Removing a large proportion of the red blood cells and plasma (liquid portion of the blood), which do not contain any stem cells.

The valuable blood components that remain after processing are tested for potency and viability. Testing for counts of CD34+ cells, Total Nucleated Cells and 7-AAD cell viability help enumerate the amount and viability of the stem cells to be cryopreserved. A cryo-preservative (DMSO) is then added. Aliquots (small samples of the cord blood) are kept for testing. An aliquot will be used prior to the sample being released for infusion. The final unit is positioned in a metal cassette and coded with a unique identifying number. Over twenty-four hours, the cells will be gradually brought from room temperature to -196C (-316F). Finally, the unit is placed in a vapor phase liquid nitrogen cryogenic storage tank for long term preservation. Our laboratory is secure and continually monitored. It is outfitted with battery and generator backup systems. The tanks are both electronically and manually monitored for proper temperature and liquid nitrogen levels.


The lab thoroughly inspects and washes the cord tissue before processing. Wharton’s jelly, the gelatinous substance within the umbilical cord, is isolated for cryopreservation. The components of the umbilical cord that do not contain stem cells are removed to prevent possible contamination. The remaining portion of the umbilical cord is then dissected into small pieces and placed into multiple sterile vials.

A test vial is set aside. If the tissue is needed, this vial will be thawed and its stem cells placed in a petri dish for several days. Afterward, sterility and confluence (the amount of the dish that is covered by cell colonies) are measured.

A cryo-preservative (DMSO solution) is added to each vial prior to freezing. Each vial is labeled with a unique identification number and placed inside a secure cryo-box. Finally, over twenty-four hours, the vials are slowly brought down from room temperature to -196C (-316F) and placed in the vapor phase liquid nitrogen storage tanks alongside the cord blood.


Placental tissue stem cells are taken from an inner layer of the placenta. This is done to best isolate and maximize the number of Mesenchymal stem cells (MSCs) collected. There are many growth factors in this area of the placenta, including amnion cells, which boost MSCs reproduction. By cryopreserving this section, AlphaCord provides its parents with the most options for future use.

Upon arrival at the lab, the placenta is thoroughly washed using an aseptic cleaning solution. This removes any residual bacteria. Once washed, the placenta is cut into small segments. These are placed into multiple vials containing an FDA-approved cryoprotectant. Each vial is labeled with a unique identification number and placed inside a secure cryo-box. The vials are and slowly cooled over twenty-four hours until they reach a temperature of -196C (-316F). Finally, they are permanently stored in a vapor phase, liquid nitrogen storage tank.

Unparalleled Quality Standards

Our lab tests your cord blood, cord tissue, and placental tissue prior to, and immediately after, processing. All are done in accordance with FDA, AABB, and AATB regulations. This is part of our continuous quality control protocols that help ensure every sample is free of contaminants and contains the most viable stem cells.

Start typing and press Enter to search

Shopping Cart

No products in the cart.